rabbit polyclonal anti human mmp2  (Cell Signaling Technology Inc)

Journal: International Journal of Molecular Sciences

Article Title: Suppressive Potential of Ethanolic Extracts of Parkia speciosa Hassk. Empty Pods Against Colon Cancer Cell Migration and Invasion

doi: 10.3390/ijms27042072

Figure Lengend Snippet: Western blot showing the effects of the PSET on the abundance of proteins associated with the downstream EMT pathway. Representative Western blot image showing the amount of MMP2, MMP9 and GAPDH proteins in control (untreated cells) and treatment (PSET) groups. The molecular weight (MW) of each protein is provided. Quantitative analysis of protein levels following Western blot analysis. Data in histograms are presented as the means ± SDs from three independent experiments ( n = 3), with asterisks indicating statistical differences: * p < 0.05.

Article Snippet: The primary antibodies used were rabbit polyclonal anti-human MMP2 (#4022), MMP9 (#3852), N-cadherin (#4061), and GAPDH (#2118) (dilution 1:500; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Western Blot, Control, Molecular Weight

Journal: Reproductive biomedicine online

Article Title: METTL3 and METTL14-mediated N 6 -methyladenosine modification promotes cell proliferation and invasion in a model of endometriosis.

doi: 10.1016/j.rbmo.2022.10.010

Figure Lengend Snippet: FIGURE 3 Validation of the N6-methyladenosine (m6A) methylation level and mRNA level of the genes enriched in estrogen signalling. (A) The m6A methylation level of MMP2, NCOA1, TLR4, PPP2R2D and YWHAZ in the ectopic endometrium (black bar) and eutopic endometrium (EU) (hatched gray bar) was validated using MeRIP quantitative polymerase chain reaction; (B) the expression of MMP2, NCOA1, TLR4, PPP2R2D and YWHAZ in the ectopic endometrium (black bar) and eutopic endometrium (hatched gray bar) using quantitative reverse transcription polymerase chain reaction; and (C) immunohistochemistry was carried out to assess the protein levels of MMP2, NCOA1, PPP2R2D and YWHAZ in the ectopic endometrium and eutopic endometrium; magnification, × 200. Two-tailed Student's t-tests were used for comparisons between ectopic endometrium and eutopic endometrium. All samples were assayed in triplicate.

Article Snippet: The polyclonal rabbit immunoglobulin G (IgG) antibody against human NCOA1 (51114-1-AP) (ProteinTech, Rosemont, IL, USA), polyclonal rabbit IgG antibody against human MMP2 (10373-2-AP) (ProteinTech, Rosemont, IL, USA), polyclonal rabbit antibody against human PPP2R2D (bs-19968P) (BiosSS Woburn, MA, USA), polyclonal rabbit IgG antibody against human YWHAZ (14881-1-AP) (Proteintech, Sankt LeonRot, Germany), were diluted at 1:1000.

Techniques: Biomarker Discovery, Methylation, Real-time Polymerase Chain Reaction, Expressing, Reverse Transcription, Polymerase Chain Reaction, Immunohistochemistry, Two Tailed Test

Journal: Reproductive biomedicine online

Article Title: METTL3 and METTL14-mediated N 6 -methyladenosine modification promotes cell proliferation and invasion in a model of endometriosis.

doi: 10.1016/j.rbmo.2022.10.010

Figure Lengend Snippet: FIGURE 5 Knockdown of METTL3, METTL14, or both, contributes to the cellular phenotype of endometriosis. (A–B) Quantitative polymerase chain reaction and Western blotting analysis of the expression of NCOA1, MMP2 For photomicrographs, please provide scale bars. PPP2R2D and YWHAZ after the knockdown of METTL3 and(or) METTL14. Cell viability assay of the cells with METTL3 and METTL14 knockdown; (C) the CCK-8 assay of the cell viability after the knockdown of METTL3 and METTL14; (D) the scratch assay of the cell migration after the knockdown of METTL3 and METTL14; (E) transwell assay of the effect of the METTL3 and METTL14 knockdown on the invasion and migration of endometrial stromal cells. NC: negative control; si-METTL14 was transfected with siMETTL14; si-METTL3 was transfected with siMETTL3; si-METTL3+si-METTL14 were transfected with si-METTL3 and si-METTL14. All samples were assayed in triplicate.

Article Snippet: The polyclonal rabbit immunoglobulin G (IgG) antibody against human NCOA1 (51114-1-AP) (ProteinTech, Rosemont, IL, USA), polyclonal rabbit IgG antibody against human MMP2 (10373-2-AP) (ProteinTech, Rosemont, IL, USA), polyclonal rabbit antibody against human PPP2R2D (bs-19968P) (BiosSS Woburn, MA, USA), polyclonal rabbit IgG antibody against human YWHAZ (14881-1-AP) (Proteintech, Sankt LeonRot, Germany), were diluted at 1:1000.

Techniques: Knockdown, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Viability Assay, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Assay, Negative Control, Transfection

Journal: Arquivos Brasileiros de Cirurgia Digestiva : ABCD

Article Title: MIR-203A-3P AND MMP-2 PROTEINS ARE HIGHLY EXPRESSED IN CIRCULATING TUMOR CELLS FROM PATIENTS WITH PANCREATIC CARCINOMA

doi: 10.1590/0102-672020210002e1628

Figure Lengend Snippet: Data of the study biomarkers, CTC detection rate, and markers in CTCs at all times of the study (n=9).

Article Snippet: Important proteins in the EMT were searched: anti-TGFβ-R1 (Cusabio 1:100, code: CSB-PA061850), anti-Mesothelin (Sigma-Aldrich 1:50, code: SAB5500143), anti-Vimentin (Cusabio 1:100, code: CSB-PA025857LA01HU), and anti-matrix metalloproteinase-2 (MMP2) (Cusabio 1:100, code: CSB-PA06879A0Rb).

Techniques:

Journal: Arquivos Brasileiros de Cirurgia Digestiva : ABCD

Article Title: MIR-203A-3P AND MMP-2 PROTEINS ARE HIGHLY EXPRESSED IN CIRCULATING TUMOR CELLS FROM PATIENTS WITH PANCREATIC CARCINOMA

doi: 10.1590/0102-672020210002e1628

Figure Lengend Snippet: Clinical and protein expression characteristics of the patients involved in this study.

Article Snippet: Important proteins in the EMT were searched: anti-TGFβ-R1 (Cusabio 1:100, code: CSB-PA061850), anti-Mesothelin (Sigma-Aldrich 1:50, code: SAB5500143), anti-Vimentin (Cusabio 1:100, code: CSB-PA025857LA01HU), and anti-matrix metalloproteinase-2 (MMP2) (Cusabio 1:100, code: CSB-PA06879A0Rb).

Techniques: Expressing